BIOMARKER QUANTITATION IN THE CLOUD

Ben Miles on 4/4/17 6:06 PM

Mesoscale Discovery (MSD) Sector S 600 integrated with the Transcriptic robotic cloud lab

Mesoscale Discovery (MSD) Sector S 600 integrated with the Transcriptic robotic cloud lab

The Transcriptic robotic cloud lab provides a great advantage for large-scale biomarker assays, delivering consistent sample to sample processing with robotic reliability, all on demand when you need it. We’ve enabled both large pharma and small biotech to quantify DNA, RNA and proteins from hundreds or thousands of samples in an automated and programmatically driven way.

In this post I want to focus on our commitment to providing access to modern tools in biomarker quantitation, and how integrating these tools with the Transcriptic robotic cloud lab not only increases accessibility to modern tools but also leverages the repeatability and reliability of an entire experimental workflow from treatment to analysis.

In the background, the robotics, software and science teams, at Transcriptic, have been collaborating on bringing the Mesoscale Discovery Sector S 600 online to all Transcriptic users. Today I’m happy to announce that we’ve made huge strides in enabling protein quantitation in high-throughput for our users and this is a big step in expanding that capability.

The Sector S 600 performs a function similar to traditional ELISA though instead leverages electrochemiluminescence (ECL) as a detection technique. There are a number of particular benefits to the Sector S 600 but one of the most compelling is the data to sample efficiency. This makes the Sector S 600 invaluable for those liquid or tissue biopsies that are low in quantity, such as plasma.

To achieve high levels of analyte quantitation per unit sample, the Sector S 600 uses a proprietary plate technology that consists of microplate wells modified with 10 capture antibody spots in each well. This spot array of capture antibodies enables up to 10-plex capture of target analytes all from the same sample volume in a single well. Once the target analytes have been captured on the spots the plate is washed and detection antibodies are added similar to an ELISA workflow. At this point, the device will sequentially read each spot and measure the resulting electrochemiluminescence (ECL).

In addition to the high data to sample efficiency, the MSD Sector S 600 also touts a 6-log dynamic range enabled by the ECL labelling technology, rendering even extremely low concentration analytes in your samples, detectable. Pre-validated panels in MSD’s V-plex line provide panels of 10 or more targets in human, mouse, or rat samples in the following areas:

  • Proinflammatory response
  • Neuroinflammation and Alzheimer’s
  • Cytokines
  • Chemokines

MSD also provides customizable plates in their U-plex line, where we can onboard your custom panel of targets for your biomarker quantitation workflow. Launching runs that use the Sector S600  from the Transcriptic web application is just like any other run, and the team produced a great interactive UI for quickly assessing the multiplexed panel data.

Transcriptic is happy to make the Mesoscale Discovery Sector S 600 immunoassay device available in the cloud.  It is fully integrated with our robotic cloud lab platform delivering seamless sample to data from tissue biopsies through to multiplexed protein quantitation outputs. The Sector S 600 sports a 6-log dynamic range perfect for low concentration analytes and up to 10plex analyte detection per sample for efficient data to sample material yields.

Protein quantitation with Mesoscale Discovery Sector S 600

  • Low sample quantity demands with up to 10-plex detection of targets
  • High-throughput in either 96 or 384 well plates
  • High sensitivity with a 6-log dynamic range
  • Multiple panels available including inflammation and Alzheimer’s relevant targets across human, mouse, and rat

Get started measuring multiplexed protein biomarkers today, by getting in touch with us or learn more in the MSD technical notes.